Coding

Part:BBa_K669002:Design

Designed by: Nathaly Montenegro   Group: iGEM11_Colombia   (2011-09-28)


CBP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal SpeI site found at 489
    Illegal PstI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal SpeI site found at 489
    Illegal PstI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal SpeI site found at 489
    Illegal PstI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal SpeI site found at 489
    Illegal PstI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence of primers: Fw gaattctcgaggccttctagagATG CTT GCC AAT ATT AAA AAA AC Rv caccgctagcTTA CTT AAC AGG TTT TAG GTC A


Source

http://www.ncbi.nlm.nih.gov/gene?term=3279341%5Buid%5D


References